Three-Dimensional Organization of Higher-Plant Chloroplast Thylakoid Membranes Revealed by Electron Tomography W
نویسندگان
چکیده
Eyal Shimoni,a Ophir Rav-Hon,b Itzhak Ohad,c Vlad Brumfeld,d and Ziv Reichb1 a Electron Microscopy Unit, Weizmann Institute of Science, Rehovot 76100, Israel b Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel c Department of Biological Chemistry, Hebrew University of Jerusalem, Jerusalem 91904, Israel d Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
منابع مشابه
Three-dimensional organization of higher-plant chloroplast thylakoid membranes revealed by electron tomography.
The light-harvesting and energy-transducing functions of the chloroplast are performed within an intricate lamellar system of membranes, called thylakoid membranes, which are differentiated into granum and stroma lamellar domains. Using dual-axis electron microscope tomography, we determined the three-dimensional organization of the chloroplast thylakoid membranes within cryo-immobilized, freez...
متن کاملReply: on Three-dimensional Models of Higher-plant Thylakoid Networks: Elements of Consensus, Controversies, and Future Experiments
Brangeon, J., and Mustárdy, L. (1979). Ontogenetic assembly of intra-chloroplastic lamellae viewed in 3-dimension. Biol Cell. 36: 71–80. Heslop-Harrison, J. (1963). Structure and morphogenesis of lamellar systems in granacontaining chloroplasts. Planta 60: 243–260. Hodge, A.J., McLean, J.D., and Mercer, F.V. (1955). Ultrastructure of the lamellae and grana in the chloroplasts of Zea mays. J. Bi...
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For more than half a century, electron microscopy has been a main tool for investigating the complex ultrastructure and organization of chloroplast thylakoid membranes, but, even today, the three-dimensional relationship between stroma and grana thylakoids, and the arrangement of the membrane protein complexes within them are not fully understood. Electron cryo-tomography (cryo-ET) is a powerfu...
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The three-dimensional (3-D) network of the granum-stroma thylakoid assembly of vascular plant chloroplasts exhibits complex structural/functional heterogeneity. A complete understanding of the ultrastructure of this assembly is critical for our understanding of thylakoid function. The prevailing historical model of thylakoid structure, based on information derived from serial section analyses o...
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